Papers of The 6th Japan Scar Workshop

3. Comprehensive Gene Expression and Bioinformatics Analyses of Keloids

Rei Ogawa, Chenyu Huang, Satoshi Akaishi, Hiko Hyakusoku
Department of Plastic, Reconstructive and Aesthetic Surgery, Nippon Medical School, Tokyo Japan.

Human true keloid tissue samples and control skins of adjacent normal skin were collected from the same individuals, and the gene expression profiles compared using human whole genome oligo microarrays without the effect of genetic disposition. Moreover, we employed the bioinformatics approach to analyze signaling pathways that were likely related to keloid formation. Total of seven keloid patients were carefully selected. Then, total RNA was extracted, and scanning by Agilent Whole Genome Oligo Microarrays was performed. After the gene expression profiles were analyzed, the bioinformatics approach was used to determine the signaling pathways that were likely related to keloid formation. The strongest enrichment in the up-regulated pathways was the fibrillar collagen category. On the other hand, the most significant enrichment in the down-regulated pathways was the category of lipid metabolic process. It was suggested that the comprehensively decreased expression of lipids, in particular the phospholipids, demonstrated their important roles in keloid pathophysiology, not only as membrane constituents, but also as actively participating in inflammation and production of messengers in intracellular signal transduction.
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