Papers of The 3rd Japan Scar Workshop

5. Analysis of helper T cells in the redness sites of keloids

Noriko Hattori1, Kazuo Kishi1, Hiroko Satoh2, Junji Takano3 and Tastuo Nakajima1

1:Department of Plastic Surgery, School of Medicine, Keio University, Tokyo, Japan
2:Department of Plastic Surgery, Tokyo Medical Center, National Hospital Organization, Tokyo, Japan
3:Department of Plastic Surgery, Social Insurance Saitama Hospital, Saitama, Japan

Before keloids invade normal skin and bulge, patients complain of redness and itching like symptoms of the skin. We have reported that inflammatory cells especially T cell subpopulations increase around the sites of redness of keloids. The aim of our study was to investigate the subsets of T cell subpopulations in the sites of redness around keloids. We investigated the distributions of Th1, Th2, Th17 and regulatory T cells among four groups of cases, that is keloids (center sites and redness sites), hypertrophic scars and normal skin (n=3). All tissues were fixed in formalin and embedded with paraffin. Sections were stained with anti-CD3, CD4, CD8, Foxp3, IFN-γ, IL-4, IL-17 antibodies and the subpopulations of each T cell were compared. Distributions of the regulatory T cells did not differ among the four groups. We found significantly larger numbers of Th17 cells and higher Th2:Th1 ratio around the sites of redness around the keloids compared with normal skin and hypertrophic scars. The balance of Th1 and Th2 plays an important role in fibrosis and influences various fibrosis diseases. The results suggest that keloid invasion may be prevented by controlling the balance between T cells in the sites of redness around keloids.
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