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5. Analysis of helper T cells in the redness sites of keloids
Noriko Hattori1, Kazuo Kishi1, Hiroko Satoh2,
Junji Takano3 and Tastuo Nakajima1
1:Department of Plastic Surgery, School of Medicine, Keio University,
Tokyo, Japan
2:Department of Plastic Surgery, Tokyo Medical Center, National Hospital
Organization, Tokyo, Japan
3:Department of Plastic Surgery, Social Insurance Saitama Hospital,
Saitama, Japan
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Before keloids invade normal skin and bulge, patients complain of
redness and itching like symptoms of the skin. We have reported that
inflammatory cells especially T cell subpopulations increase around the
sites of redness of keloids. The aim of our study was to investigate the
subsets of T cell subpopulations in the sites of redness around keloids.
We investigated the distributions of Th1, Th2, Th17 and regulatory T
cells among four groups of cases, that is keloids (center sites and
redness sites), hypertrophic scars and normal skin (n=3). All tissues
were fixed in formalin and embedded with paraffin. Sections were stained
with anti-CD3, CD4, CD8, Foxp3, IFN-γ, IL-4, IL-17 antibodies and the
subpopulations of each T cell were compared. Distributions of the
regulatory T cells did not differ among the four groups. We found
significantly larger numbers of Th17 cells and higher Th2:Th1 ratio
around the sites of redness around the keloids compared with normal skin
and hypertrophic scars. The balance of Th1 and Th2 plays an important
role in fibrosis and influences various fibrosis diseases. The results
suggest that keloid invasion may be prevented by controlling the balance
between T cells in the sites of redness around keloids. |
